The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 27, 2000
Filed:
May. 12, 1998
Applicant:
Inventors:
Mads Aage Laustsen, Lyngby, DK;
Stig Nielsson, Lynge, DK;
Assignee:
Novo Nordisk A/S, Bagsvaerd, DK;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ;
U.S. Cl.
CPC ...
435184 ; 435183 ; 435814 ;
Abstract
A method is presented for inactivating undesired acid labile proteases which are expressed into the culture medium simultaneously with desired enzymes by Aspergillus species when Aspergillus species is used as the host for recombinantly producing enzymes. The method involves incubating culture medium which contains a mixture of the desired recombinantly produced enzyme and the undesired acid labile protease which have been expressed therein at pH values below 4.5 and at temperatures from 2.degree. C. to 75.degree. C. for at least 20 seconds.