The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 20, 2000

Filed:

Nov. 19, 1997
Applicant:
Inventors:

Roger Y Tsien, La Jolla, CA (US);

S James Remington, Eugene, OR (US);

Andrew B Cubitt, San Diego, CA (US);

Roger Heim, Del Mar, CA (US);

Mats F Ormo, Huddinge, SE;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07H / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435325 ; 435 691 ; 4352523 ; 43525233 ; 43525411 ; 435410 ; 4353201 ; 536 231 ; 536 235 ; 530350 ;
Abstract

This invention provides functional engineered fluorescent proteins with varied fluorescence characteristics that can be easily distinguished from currently existing green and blue fluorescent proteins. In one aspect, the invention provides nucleic acids, expression vectors and recombinant host cells comprising nucleotide sequences encoding functional engineered fluorescent proteins comprising aromatic substitutions at position 66 and a folding mutation. In one embodiment the invention provides for fluorescent proteins containing an aromatic substitution at Thr 203.


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