The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 02, 2000
Filed:
Aug. 05, 1998
Moris L Silber, Moscow, ID (US);
Bruce B Davitt, Pullman, WA (US);
Washington State University Research Foundation, Pullman, WA (US);
Abstract
A rapid and simple method for making a hydrophobically associated, Coomassie Brilliant Blue (CBB) dye/protein complex comprising CBB and a protein, and a method for detecting analytes using the CBB dye/protein complex are disclosed. The method for making the CBB dye/protein complex is comprised of mixing a protein with a non-alcoholic aqueous CBB solution having a pH greater than 4, heating the mixture to a first temperature for a short pulse period, then cooling to a second temperature to form a solution containing the CBB dye/protein complex. In the method for detecting analytes that bind a CBB dye/protein complex, the analyte sample is added to the solution containing a CBB dye/protein complex to form a supernatant and a precipitate containing an analyte-bound fraction. The precipitate is dissolved in an assay solvent, and a spectral absorption of both the precipitate and supernatant is measured.