The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 11, 2000
Filed:
Nov. 12, 1996
Kevin Gary Korth, Appleton, WI (US);
Sarah Elizabeth Heathcock, Menasha, WI (US);
Linda Susan Huard, Appleton, WI (US);
Kimberly-Clark Corporation, Neenah, WI (US);
Abstract
The present invention relates to a method for detecting the presence of P. aeruginosa in a fluid sample. The method uses PCR to amplify the expression of a segment of the exotoxin A gene sequence. The method includes treating strands of nucleotide fragments with a first oligonucleotide primer and a second oligonucleotide primer. The primers are sufficiently complementary to the fragment to hybridize a region having from about 400 to 1200 base pairs. Desirably, the first oligonucleotide primer includes the sequence 5'-ACA ACG CCC TCA GCA TCA CCA-3' and the second oligonucleotide primer includes the sequence 5'-CGG GTC GAG CAG GCA CAA C-3'.