Multi parameter scanner

Abstract

An apparatus capable of measuring quantities of biological or other types of samples that have been labeled using any of a variety of techniques including fluorescence, radioisotopes, enzyme activated light emitting chemicals, and enzyme activated fluorescent materials is provided. The apparatus allows for either simultaneous or sequential acquisition of signals from multiple sample types. The apparatus is not restricted to a particular source or wavelength of excitation or readout light, nor is the apparatus restricted to a particular emission wavelength. The provided scanner includes a source module that preferably contains an internal laser emitting two different wavelengths of approximately the same intensity. An optional external light source may be coupled to the source module, thus adding further flexibility through the addition of other wavelengths (e.g., V, visible, mid-IR, and IR). The scanner also includes a detection module. Within the detection module are two detectors, thus allowing the simultaneous detection of multiple wavelengths. A bifurcated optical cable is used to transfer the excitation and/or readout light from the source module to the sample and subsequently transfer the emitted and/or scattered light from the sample to the detection module. The scanning stage of the scanner is designed to accommodate a variety of samples, ranging from phosphor screens, gels, and fluorescent samples to microtiter plates. An internal microprocessor is used to control the various aspects of the scanner, preferably including translation stage control, source filters, and detection filters. The internal microprocessor may be coupled to an external computer. The external computer may be used to change the programming of the microprocessor, provide a user interface to the microprocessor, process and store test results, and display sample images.