The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 22, 2000
Filed:
Dec. 20, 1996
Hermann Leying, Bichl, DE;
Matthias Hinzpeter, Munchen, DE;
Heiko Wittor, Tutzing, DE;
Hans-Peter Fritton, Morlenbach, DE;
Roche Diagnostics GmbH, Mannheim, DE;
Abstract
Method and kit for the quantitative detection of specific oligonucleotide or polynucleotide sequences which is characterized in that a sample mixture containing RNA or single-stranded DNA is hybridized with an oligonucleotide or polynucleotide probe(s) which are complementary to the nucleotide sequence to be determined and carry(ies) a specificplly bindable and a detectable chemical group subsequently it is admixed with an agent that cleaves single-stranded polynucleotide sequences and the immobilized or non-immobilized nucleotide probe is determined after transfer into a suitable reaction vessel. It has proven to be particularly advantageous when a mixture is used which is composed of different cleaving reagents.