The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 08, 2000
Filed:
Aug. 26, 1996
Alexander Steinbuchel, Altenberge, DE;
Ursula Pieper-Furst, Gottingen, DE;
Monsanto Company, St. Louis, MO (US);
Abstract
The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M.sub.r 15,500 protein of Rhodococcus ruber, which is referred to as the GA14-protein, was analysed. The sequence revealed that the corresponding structural gene is represented by the open reading frame 3 encoding a protein with a calculated M.sub.r 14,175 which was recently localized downstream of the PHA synthase gene (Pieper, U., and A. Steinbuchel, 1992. FEMS Microbiol. Lett. 96: 73-80). A recombinant strain of Escherichia coli XL1-Blue carrying the hybrid plasmid (pSKXA10*) with ORF3 overexpressed the GA14-protein. The GA14-protein was subsequently purified in a three-step procedure including chromatography on DEAE-Sephacel, Phenyl-Sepharose CL-4B and Superose 12. Determination of the molecular weight by gel filtration as well as electron microscopic studies make a tetrameric structure of the recombinant, native GA14-protein most likely. Immunoelectron microscopy demonstrated a localization of the GA14-protein at the periphery of PHA granules as well as close to the cell membrane in R. ruber. Investigations of PHA-leaky and PHA-negative mutants of R. ruber indicated that the expression of the GA14-protein depended strongly on PhA synthesis.