The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 11, 2000
Filed:
Feb. 20, 1998
Applicant:
Inventor:
Jang B Rampal, Yorba Linda, CA (US);
Assignee:
Beckman Coulter, Inc., Fullerton, CA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07H / ;
U.S. Cl.
CPC ...
536 253 ; 435-6 ; 422 50 ; 422 681 ;
Abstract
Disclosed herein is a method for attaching pre-synthesized oligonucleotides to a polypropylene support medium. Most preferably, a polypropylene film is aminated by a plasma discharge in the presence of ammonia gas. An oligonucleotide having a terminal phosphate is activated in the presence of an imidazole and a carbodiimide to form a phosphorimidazolide. The activated oligonucleotide becomes immobilized by forming a phosphoramidate bond with the aminated polypropylene. The invention can be used to construct oligonucleotide arrays for hybridization assays.