The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 04, 2000

Filed:

Jul. 17, 1997
Applicant:
Inventor:

Yasunori Kihira, Shiga-ken, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C07H / ;
U.S. Cl.
CPC ...
435226 ; 435212 ; 435219 ; 4352523 ; 43525233 ; 4353201 ; 536 231 ; 536 232 ; 536 235 ;
Abstract

The present invention relates to a process for producing a human matrilysin characterized in that a human promatrilysin is expressed in E. coli and secreted into the periplasm thereof; the inclusion body is formed, the human promatrilysin is solubilized with a urea solution, purified and renatured to obtain the active enzyme (active-type matrilysin). By this process, the active human matrilysin can be easily produced from transformed E. coli.


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Kihira, Yasunori. (2000). Process for producing human matrilysin by means of recombinant dna (U.S. Patent No. 6010893). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/6010893

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