The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 21, 1999
Filed:
May. 16, 1995
Biotechnology Australia Pty Limited, New South Wales, AU;
Abstract
A method is provided for the detection of low levels of a microorganism in a sample in the presence of competing microflora, comprising the steps of: (a) exposing the sample to a solid support to which are adsorbed antibodies specific for said microorganism; (b) washing the solid support to remove unbound material; (c) combining the solid support with sterile nutrient broth which permits replication of said microorganism; (d) incubating the solid support with said nutrient broth at a temperature and for a time sufficient to allow said microorganism to replicate and be recaptured by said antibodies on said solid support; (e) releasing the bound microorganism from the solid support into a solution; and (f)assaying the solution for the microorganism. A kit for performing the assay also is provided. This kit comprises a solid support to which are adsorbed antibodies specific for the microorganism, a first container comprising a washing solution, a second container comprising a sterile nutrient broth, and, optionally, a third container comprising a solution comprising a releasing agent.