The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 14, 1999
Filed:
Apr. 14, 1998
Applicant:
Inventors:
Gene G-Y. Shen, Diamond Bar, CA (US);
Thomas S Dobashi, Rosemead, CA (US);
Assignee:
Beckman Instruments, Inc., Fullerton, CA (US);
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07F / ; C07F / ; C07D / ; C07D / ;
U.S. Cl.
CPC ...
544232 ; 544277 ; 544317 ; 546 23 ; 546 24 ; 548113 ; 548547 ; 548548 ; 548549 ; 548579 ; 548565 ; 548453 ; 549473 ;
Abstract
Activating groups for cyanine dyes used to label chain terminators in nucleotide sequencing, based on N-hydroxyphthalimide, are disclosed. From these activating groups, activated dyes of the present Invention are prepared which react with the derivitized nucleotide chain terminators to give a labeled chain terminator of the present Invention. The activating groups of the present Invention allow the dye-chain terminator reaction to occur at a much higher yield and with much greater selectivity for the mono-substituted product, compared with the prior art.