The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 16, 1999
Filed:
Feb. 28, 1997
Applicant:
Inventors:
Uffe Mortensen, K.o slashed.benhavn S, DK;
Kjeld Olesen, Frederiksberg, DK;
Henning Stennicke, San Diego, CA (US);
Steen B So slashed.rensen, Solr.o slashed.dstrand, DK;
Klaus Breddam, Roskilde, DK;
Assignee:
Carlsberg Laboratory, Copenhagen Valby, DK;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435129 ; 435195 ; 435212 ; 435223 ; 435224 ; 435440 ; 530402 ;
Abstract
A method for transamidating a peptide substrate having a P.sub.1 amino acid residue with a positively charged side chain. According to the invention, carboxypeptidase Y is modified to substitute at least one amino acid having a negatively charged side chain in an S.sub.1 subsite. Additionally, the modified carboxypeptidase Y can include substituted amino acid residues in an S.sub.1 ', S.sub.2 and/or S.sub.3 subsite to accommodate a specific peptide substrate.