The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 02, 1999
Filed:
Aug. 22, 1996
Applicant:
Inventors:
Allan Svendsen, Birker.o slashed.d, DK;
Ib Groth Clausen, Hiller.o slashed.d, DK;
Jens Sigurd Okkels, Frederiksberg C, DK;
Marianne Thellersen, Frederiksberg C, DK;
Assignee:
Novo Nordisk A/S, Bagsv.ae butted.rt, DK;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C07H / ;
U.S. Cl.
CPC ...
435198 ; 435 691 ; 4352523 ; 4353201 ; 435471 ; 435832 ; 435849 ; 435874 ; 435886 ; 435913 ; 435911 ; 536 232 ;
Abstract
The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.