The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 26, 1999

Filed:

Nov. 19, 1997
Applicant:
Inventors:

Richard H Aster, Milwaukee, WI (US);

Gian Visentin, Shorewood, WI (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; G01N / ; G01N / ;
U.S. Cl.
CPC ...
436503 ; 427-213 ; 435-71 ; 435-72 ; 435-79 ; 435-792 ; 435-794 ; 435 13 ; 435 28 ; 435961 ; 435970 ; 435975 ; 436501 ; 436503 ; 436513 ; 436518 ; 436529 ; 436174 ; 436175 ; 436176 ; 436530 ; 436531 ; 436532 ; 436808 ;
Abstract

A method of detecting heparin-induced antibodies to complete a diagnosis of heparin-induced thrombocytopenia (HITP) is disclosed. This method comprises the first step of attaching a glycosaminoglycan to a solid support, wherein the glycosaminoglycan is attached to the solid support only at the reducing end of the molecule (unidirectionally). Platelet factor 4 is then bound to the glycosaminoglycan forming a complex having an epitope recognizable by antibodies generated in an HITP immune response. Human blood plasma or serum from a patient suspected of having HITP is exposed to the complex and the complex is analyzed to determine if HITP-related antibodies are present. A device and kit used in performing the diagnostic assay are also disclosed.


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