The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 19, 1999
Filed:
Oct. 09, 1997
Hitachi, Ltd., Tokyo, JP;
Abstract
The present invention provides a DNA sequencing method comprising: (1) a step of fragmentation of a sample DNA and amplifying each fragment to obtain a first DNA fragment; (2) a step of obtaining from the first DNA fragment a second DNA fragment substantially complementary to the sample DNA at least at the 3' terminus thereof; and (3) a step of performing an extension reaction of complementary strand, using the sample DNA as a template to produce a third DNA fragment containing a base sequence complementary to the second DNA fragment and having a size longer than that of the second DNA fragment, and using the third DNA fragment as a template for sequencing of the sample DNA. DNA sequencing can be proceeded efficiently with extremely low redundancy.