The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 28, 1999
Filed:
Mar. 14, 1997
Applicant:
Inventors:
Ole Thastrup, Birker.o slashed.d, DK;
So slashedren Tullin, S.o slashed.borg, DK;
Lars Kongsbak Poulsen, Holte, DK;
Sara Petersen Bjo slashed.rn, Lyngby, DK;
Assignee:
Novo Nordisk A/S, Bagsvaerd, DK;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12N / ; C12N / ; C07K / ;
U.S. Cl.
CPC ...
435-74 ; 435-71 ; 435-77 ; 435 691 ; 435183 ; 4353201 ; 435325 ; 4352523 ; 43525411 ; 536 231 ; 536 235 ; 536 234 ;
Abstract
The present invention provides a method for detecting a biologically active substance that affects intracellular processes mediated through a protein kinase or second messenger, which comprises incubating a cell with a test substance and measuring any change caused by the test substance in the fluorescence of (i) green fluorescent protein (GFP) having a protein kinase recognition site or (ii) GFP that has been modified to contain a second messenger binding domain.