The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 28, 1999
Filed:
Jan. 15, 1998
Applicant:
Inventor:
Alexander Mankin, Oak Park, IL (US);
Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435-6 ;
Abstract
An easily automated method of screening for antibiotics active against erythromycin resistant strains, comprising footprinting an antibiotic on the domain II of 23S rRNA, isolating rRNA by incubating an antibiotic with ribosomes, modifying ribosomes with a chemical modifying agent, isolating modified rRNA and subjecting it to the reverse transcriptase-mediated primer extension and gel electrophoresis on a DNA sequencer to determine the extent of antibiotic-induced protection of an rRNA nucleotide from chemical modification.