The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 07, 1999

Filed:

Jun. 06, 1995
Applicant:
Inventors:

Brian K Kay, Chapel Hill, NC (US);

Dana M Fowlkes, Chapel Hill, NC (US);

Nils B Adey, Carrboro, NC (US);

Andrew B Sparks, Carrboro, NC (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 691 ; 435 697 ; 435-71 ; 435471 ; 536 234 ;
Abstract

A novel method for producing novel and/or improved heterofunctional binding fusion proteins termed Totally Synthetic Affinity Reagents (TSARs) is disclosed. TSARs are concatenated heterofunctional proteins, polypeptides or peptides comprising at least two functional regions: a binding domain with affinity for a ligand and a second effector peptide portion that is chemically or biologically active. In one embodiment, the heterofunctional proteins, polypeptides or peptides further comprise a linker peptide portion between the binding domain and the second active peptide portion. The linker peptide can be either susceptible or not susceptible to cleavage by enzymatic or chemical means. Novel and/or improved heterofunctional binding reagents as well as methods for using the reagents for a variety of in vitro and in vivo applications are also disclosed.


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