The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 24, 1999

Filed:

Nov. 12, 1998
Applicant:
Inventors:

Michael W Hunkapiller, San Carlos, CA (US);

Andrew C Hiatt, San Diego, CA (US);

Assignee:

The Porkin-Elmer Corporation, Foster City, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H / ;
U.S. Cl.
CPC ...
536 253 ; 435-6 ; 435 912 ; 436501 ; 935 77 ; 935 78 ;
Abstract

The present invention concerns methods of assembly of a polynucleotide on a solid-support by performing steps of annealing, ligation, and extension. The steps may be repeated in a cyclical manner to assemble immobilized double- or single-stranded polynucleotides with functional gene properties. The immobilized polynucleotides may be amplified by the polymerase chain reaction, and detected and quantitated by an exonuclease assay with a self-quenching, fluorescent probe. The polynucleotide may be cleaved from the solid-support by chemical or enzymatic cleavage.


Find Patent Forward Citations

Hunkapiller, Michael. (1999). Ligation assembly and detection of polynucleotides on solid-support (U.S. Patent No. 5942609). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/5942609

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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