The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 03, 1999
Filed:
Jan. 12, 1998
Applicant:
Inventors:
David C Wyllie, Cranford, NJ (US);
John Chu-Tay Tang, Livingston, NJ (US);
Assignee:
Schering Corporation, Kenilworth, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D / ;
U.S. Cl.
CPC ...
210635 ; 210656 ; 530413 ; 530417 ;
Abstract
A method for purifying protein containing histidine residues using immobilized metal, affinity chromatography. The hydrophilic index of the histidine residues is determined (HI). If the HI is at least 2 the pH of the solution containing the protein is adjusted to about 6.75 to 7.2 and applied to the IMAC column such that the protein binds to the column.