The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 27, 1999
Filed:
Dec. 15, 1995
Applicant:
Inventors:
Fumio Imamoto, Osaka, JP;
Yoshizumi Ishino, Shiga, JP;
Mitsuru Furusawa, Tokyo, JP;
Hirofumi Doi, Kanagawa, JP;
Assignee:
Research Development Corporation of Japan, Saitama, JP;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435-6 ; 435440 ; 435441 ; 435449 ; 435448 ;
Abstract
This invention provides A method for preparing mutant genes, which comprises the steps of constructing a recombinant plasmid DNA by inserting a gene fragment into a plasmid DNA having a unidirectional origin, introducing the recombinant plasmid DNA into host cell lacking DNA error-correcting function to transform the host cell, and culturing the transformant cell in a condition that any mutations of the inserted gene is detectable. According to the present invention, diverse mutant genes can be prepared in a short period of time.