Soft x-ray microfluoroscope

Abstract

A plasma source of soft x-rays provides the illumination for a microfluoroscope. In general, an x-ray relay optic collects part of the diverging plasma radiation and redirects it to a distant plane. At that plane, the fine-grained or grainless fluorescent screen of a microfluoroscope is placed to receive the radiation. A specimen is placed in direct contact with the screen, or in very close proximity, so that its x-ray shadow is projected onto the screen. The screen is very thin and transparent to visible or ultraviolet light so that a high-numerical-aperture optical microscope objective can closely approach and view the screen from the opposite side. The optical microscope views the fluorescent light emitted by the screen, which corresponds to the x-ray absorption shadow of the specimen. In general, a very thin, x-ray transparent vacuum window is used to separate the specimen, fluorescent screen, and microscope from the vacuum of the plasma source. Thin-film filters and/or monochromator devices are used to limit the wavelengths of soft x-rays which reach the fluorescent screen to the desired energy range. The use of the apparatus and process occurs with either a separate instrument or as an add-on feature to a conventional optical microscope.