The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 08, 1999
Filed:
Sep. 06, 1996
Kay Lichtenwalter, San Jose, CA (US);
Calvin B Ward, Castro Valley, CA (US);
Hewlett-Packard Company, Palo Alto, CA (US);
Abstract
A method for detecting the presence of a target nucleic acid species having first, second and third target sequences. In the present invention, a solution to be tested is brought into contact with a solid support having a probe nucleic acid species attached thereto, the probe nucleic acid sequence comprising a sequence that is complementary to the first target sequence. A dye solution is then brought into contact with the solid support. The dye solution includes a first dye attachment nucleic acid sequence coupled to a first dye and a second dye attachment nucleic acid sequence coupled to a second dye. The first dye attachment nucleic acid sequence is complementary to the second target sequence and the second dye attachment nucleic acid sequence is complementary to the third target sequence. The ratio of concentrations of the first and second dye attachment nucleic acid sequences in the dye solution is different from the ratio of the first and second dye attachment sequences specifically bound by the target nucleic acid species. By measuring the ratio of the first and second dyes, non-specific binding of the dye attachment sequences can be detected. In one embodiment of the invention, the target nucleic acid sequence comprises a fourth target sequence, and the dye solution includes a third dye attachment nucleic acid sequence complementary to the fourth target sequence and coupled to a third dye.