The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 11, 1999

Filed:

Dec. 05, 1997
Applicant:
Inventors:

Joseph L Di Cesare, Redding, CT (US);

Steven M Rosen, Mountain Lakes, NJ (US);

Assignees:

The Perkin-Elmer Corporation, Norwalk, CT (US);

Roche Diagnostic Systems, Inc., Branchburg, NJ (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435-4 ; 435-5 ; 435-731 ; 435-732 ; 435-733 ; 435-735 ; 435-736 ; 435-737 ; 436518 ;
Abstract

The presence or absence of an organism in a sample is detected by isolating the organism from the sample by a suitable affinity matrix, releasing the organism from the affinity matrix, rupturing the cells of the organism to release total nucleic acid and hydrolyzing or digesting the total nucleic acid to form mononucleotides or individual free nucleic acid basic and inorganic phosphate to form an analyte solution, and assaying the analyte solution for the at least one presence of free nucleic acid base or inorganic phosphate to thereby determine whether the organism was present in the sample.


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