The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 04, 1999
Filed:
Mar. 03, 1997
Paul Richard Gotti, Albuquerque, NM (US);
Trenton Todd Rees, Albuquerque, NM (US);
Syncor International Corporation, Woodland Hills, CA (US);
Abstract
Disclosed is a method for separating leukocytes from platelets. Blood serum is added to a centrifuge tube. A suspension containing leukocytes and platelets is then added to form a layer on top of the blood serum, the layer having a density less than the density of the blood serum. The cells and the layers are centrifuged at a force and for a time sufficient to separate the leukocytes from the platelets. The resulting, isolated leukocytes are then recovered from the centrifuge tube. In some embodiments, a suspension of the leukocyte cells are pretreated by incubating with a stannous ion reducing agent, the suspension having a stannous ion concentration of from about 50 .mu.g to about 1000 .mu.g stannous ion per 10.sup.8 leukocyte cells. A suspension of the pretreated leukocyte cells is then incubated with a solution containing a technetium-99m (VII) salt, preferably sodium .sup.99m Tc-pertechnetate, to radiolabel the leukocyte cells.