The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 04, 1999

Filed:

Sep. 01, 1995
Applicant:
Inventors:

Gunther Eberz, Leverkusen, DE;

Hans-Georg Rast, Bergisch Gladbach, DE;

Assignee:

Bayer Aktiengesellschaft, Leverkusen, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 37 ; 4351721 ; 4352523 ;
Abstract

The method of producing luminescent bacteria is based on the transfer of genetic information for biological luminescence to obligate chemolithoautotrophic nitrifying microorganisms. In particular, luciferase DNA or a luciferase-beta-galactosidase transcription fusion is transferred into ammonia-oxidizing bacteria with the aid of plasmid- or transposon vector systems. The bioluminescent nitrification microorganisms, particularly Nitrosomonas strains modified by genetic engineering, are used in order to identify specific inhibitors during nitrification processes, the viability of these microorganisms being determined by measuring the decrease in luminescence. A particularly important application is that inhibitors in a nitrifying biological sewage treatment plant can be detected in this manner.


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