The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 30, 1999

Filed:

May. 03, 1996
Applicant:
Inventor:

Michael Joseph Kuranda, Acton, MA (US);

Assignee:

Millennium Pharmaceuticals, Inc., Cambridge, MA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; G01N / ;
U.S. Cl.
CPC ...
435 15 ; 435-72 ; 435-731 ; 435-732 ; 435-791-7.95 ; 435 97 ; 435 14 ; 435-4 ; 435810 ; 435 692 ; 435100 ; 435101 ; 435105 ; 435183 ; 435223 ; 435224 ; 435329 ; 514-1 ; 514 23 ; 514 54 ; 514 55 ; 436 89 ; 436 92 ; 436 94 ; 530370 ; 530371 ; 5303871 ; 5303875 ;
Abstract

Disclosed are methods that can be used to (1) measure the level of polysaccharide in a sample; (2) measure the ability of a compound to degrade a polysaccharide; (3) measure the ability of a compound to modulate polysaccharide synthesis; and (4) identify or distinguish a polysaccharide, and hence organism, for diagnostic purposes in clinical medicine or research. The invention stems from Applicant's discovery that polysaccharides have multiple binding sites for polysaccharide binding moieties (PBM, e.g., wheat germ agglutinin (WGA)). In each method, one PBM links the polysaccharide to a substrate, and a tagged PBM is used to detect the polysaccharide. All of these methods can be carried out rapidly and quickly in the wells of a microtiter plate, thus permitting high through-put screening of samples or test compounds.


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