The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 23, 1999
Filed:
Sep. 14, 1992
Applicant:
Inventors:
Ohji Ifuku, Yokohama, JP;
Shinitiro Haze, Yokohama, JP;
Jiro Kishimoto, Yokohama, JP;
Kazuo Nakahama, Nagaokakyo, JP;
Assignee:
Shiseido Company, Ltd., Tokyo, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 691 ; 4351723 ; 43525233 ; 4353201 ; 536 231 ; 536 237 ;
Abstract
A DNA sequence of high biotin operon-expression system usable for breeding a bacterium having excellent biotin productivity is provided. A DNA sequence of biotin operon characterized in the fact that at least one base pair of either a nucleotide sequence of the regulatory region of the biotin operon of Escherichia coli or a nucleotide sequence in the vicinity of the bioB initiating codon is mutated in comparison with that of the one in its wild type strain is provided. Escherichia coli transformed with a recombinant plasmid carrying such a DNA sequence has high biotin-productivity.