The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 02, 1999
Filed:
May. 15, 1995
Applicant:
Inventors:
Richard J Jones, Bel Air, MD (US);
O Michael Colvin, Baltimore, MD (US);
John Hilton, Parkton, MD (US);
Saul Sharkis, Towson, MD (US);
Assignee:
Johns Hopkins University School of Medicine, Baltimore, MD (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435 26 ; 435 29 ; 435372 ;
Abstract
Production of a stem cell enriched cell composition using essentially non-toxic methodology comprising contacting a cell mixture with a cell-permeable, fluorescent non-polar compound that reacts upon contact with intracellular enzyme aldehyde dehydrogenase or phosphokinase found substantially exclusively in stem cells so as to form a biocompatible fluorescent product that is polar and non-permeable to the membrane of the stem cells, and isolating the cells containing the marker by fluorescent cell sorting.