The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 23, 1999

Filed:

Nov. 26, 1997
Applicant:
Inventors:

Elizabeth Anne Weavers, Aylesbury, GB;

Michael Joseph Powell, Gaithersburg, MA (US);

Assignee:

Alusuisse Holdings A.G., Neuhausen am Rheinfall, CH;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; G01N / ;
U.S. Cl.
CPC ...
435 28 ; 435-4 ; 435975 ; 536 51 ; 536-11 ; 534822 ; 514 59 ;
Abstract

We have discovered that the stability of a dilute peroxidase-containing solution is greatly enhanced by the addition of a specific substrate for the peroxidase, in the absence of peroxide. We provide a peroxidase-containing reagent consisting of a buffered aqueous solution comprising a peroxidase or a peroxidase conjugate and a specific substrate for the peroxidase, in the absence of peroxide. The peroxidase may be a free peroxidase but for assay purposes is preferably a peroxidase bound to a specific binding component of an assay, to form a peroxidase conjugate. The peroxidase conjugate is preferably a conjugate between a peroxidase and an antigen or an antibody, most preferably an antibody. The peroxidase is preferably horseradish peroxidase.


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