The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 16, 1999
Filed:
Dec. 13, 1994
Robert Charles Boraston, Carshalton Beeches, GB;
Alusuisse Holdings A.G., , CH;
Abstract
Adherent animal cells are grown in suspension in a nutrient medium in which the molar ratio of total inorganic ions and total amino acids is reduced when compared with the corresponding ratio in known nutrient media, and is maintained at a level at which little or no cell aggregation occurs. Further, the nutrient medium contains 10:1 to about 1:1 molar ratio of total inorganic ions to total amino acids. In one further embodiment, the nutrient medium contains a total sodium ion concentration in the range of 75 to 120 mM (millimole), a total chloride ion concentration in the range of 50 to 90 mM and a total amino acid content of 20 to 50 mM. The adherent animal cells include mammalian cells such as cells belonging to a human, rat, mouse or hamster. Chinese Hamster Ovary Cells (CHO cells) are cultured using the process of the disclosed invention.