The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 16, 1999

Filed:

Jun. 20, 1996
Applicant:
Inventors:

Hirokazu Matsukawa, Osaka-fu, JP;

Tuyosi Fujita, Osaka-fu, JP;

Tairo Oshima, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 26 ; 435 25 ; 435189 ; 435190 ; 435822 ; 435966 ;
Abstract

The invention relates to an ammonia elimination reagent for use in an enzymatic assay of a biological substance which produces ammonia as the reaction product. The reagent is an alkaline solution of pH 9 to 11 and contains Sulfolobus-derived thermostable isocitrate dehydrogenase, 2-oxoglutaric acid, a reducing coenzyme, isocitric acid, glutamate dehydrogenase, and a divalent metal salt. The reagent can be stored in solution and has excellent stability under conditions of alkaline pHs.


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