The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 09, 1999
Filed:
Nov. 25, 1996
Applicant:
Inventors:
David M Schuster, Poolesville, MD (US);
Ayoub Rashtchian, Gaithersburg, MD (US);
Assignee:
Life Technologies, Inc., Rockville, MD (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ;
U.S. Cl.
CPC ...
435-6 ; 435 912 ; 435 9152 ;
Abstract
The present invention provides improved methods for amplifying a nucleic acid molecule. More specifically, the invention involves replacing at least one nucleotide of an oligonucleotide with a nucleotide having altered base pairing characteristics, so as to more equalize the efficiency with which that oligonucleotide and a second oligonucleotide hybridize to a target molecule and then amplifying the target molecule using, for example, the polymerase chain reaction. Improved amplification results from the improvement in relative hybridization efficiencies.