The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 26, 1999

Filed:

Nov. 01, 1996
Applicant:
Inventors:

C Peter Romaine, State College, PA (US);

Gary W Moorman, Port Matilda, PA (US);

Michael A Sulzinski, Scranton, PA (US);

Assignee:

The Penn State Research Foundation, University Park, PA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 435 912 ; 4352523 ; 4353201 ; 536 2432 ; 536 2433 ; 536 253 ;
Abstract

This invention provides methods and reagents for screening plants, particularly geraniums, for infections with bacterial pathogens. Specifically, the invention provides nucleic acid amplification and detection methods for specifically detecting a certain pathovar of a bacterial infectious agent in geraniums. The invention provides a nucleic acid hybridization probe that is specific for Xanthomonas campestris pathovar pelargonii, and paired sets of oligonucleotide primers for polymerase chain reaction-catalyzed amplification of DNA fragments comprising the nucleic acid sequences of the invention specific for Xanthomonas campestris pathovar pelargonii template DNA. The invention also provides PCR-based methods for detecting Xanthomonas campestris pathovar pelargonii DNA, and in particular for distinguishing pathovar DNA from the DNA of the host infected plant. Methods for producing detectably-labeled hybridization probes using PCR are also provided.


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