The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 12, 1999

Filed:

Sep. 25, 1996
Applicant:
Inventors:

Shawn P Cain, North Chelmsford, MA (US);

Timothy J Perlman, Lexington, MA (US);

Deborah J Deane, Orange, MA (US);

Claudy J-P Mullon, Framingham, MA (US);

Assignee:

W.R. Grace & Co.-Conn., Lexington, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ;
U.S. Cl.
CPC ...
435-2 ; 435-11 ; 435325 ;
Abstract

The invention features a method of processing cells in a closed system that results in a suspension of cells in a transfer vessel containing a target number of cells. The number of cells in the closed vessel is determined from the cell concentration (i.e., the number of viable cells/mL) in the closed vessel and the total volume of the suspension in the closed vessel. The volume of the suspension in the closed vessel can be determined from the weight of the suspension and its density. In particular, the cells are preserved in a protective medium and are recovered substantially free of the protective medium in a closed vessel containing the known number of cells in a suspension.


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