The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 05, 1999
Filed:
Feb. 23, 1996
Min Li, Lutherville, MD (US);
The Johns Hopkins University School Of Medicine, Baltimore, MD (US);
Abstract
A polypeptide consisting essentially of the NAB and linking region of an .alpha.-subunit of Shaker-like potassium ion channel which binds to a core region of a .beta.-subunit of said Shaker-like potassium ion channel. A related polypeptide is also provided and consists essentially of the core region of a .beta.-subunit of a Shaker-like potassium ion channel which binds to the NAB and linking region of an .alpha.-subunit of said Shaker-like channel. Nucleic acid sequences which encode these polypeptides, vectors containing those sequences, expression systems, hosts cells containing the aforesaid polypeptides, and pharmaceutical formulations of the peptides are also provided. Other aspects of the invention include methods of modulating the flow of potassium ions through a cell membrane surrounding a cytoplasm by introducing either of the aforesaid polypeptides or exogenous Kv.beta.2 protein into the cytoplasm of the cell, methods of detecting a molecule that binds to either of the aforesaid polypeptides, and an improved yeast two-hybrid system which utilizes the aforesaid polypeptides.