The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 03, 1998

Filed:

Mar. 28, 1997
Applicant:
Inventors:

Satoshi Fujita, Nisshin, JP;

Naoto Kagiyama, Sapporo, JP;

Masayoshi Momiyama, Sapporo, JP;

Yasumitsu Kondoh, Sapporo, JP;

Miho Nishiyauchi, Sapporo, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; G01N / ; C07H / ; C07K / ;
U.S. Cl.
CPC ...
435-6 ; 435 21 ; 435 681 ; 435968 ; 436 86 ; 436 87 ; 436 89 ; 436800 ; 536 243 ; 530350 ;
Abstract

A method of detecting nucleic acids, proteins, or protein nucleic acid complexes. The method includes binding an enzyme, such as phosphatase, to a specimen of the nucleic acid, protein, or protein nucleic acid complex. The enzyme is then reacted with a fluorescein derivative phosphate ester to obtain a fluorescein derivative phosphate ester hydrolysate. The hydrolysate is then irradiated with excitation light, and the emitted fluorescein is detected.


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