The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 27, 1998
Filed:
Feb. 28, 1996
Applicant:
Inventors:
Kemp B Cease, Ann Arbor, MI (US);
Cortland J Lohff, Madison, WI (US);
Assignee:
The Regents Of The University Of Michigan, Ann Arbor, MI (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ;
U.S. Cl.
CPC ...
4351723 ; 4353201 ; 536 231 ; 536 242 ;
Abstract
Plasmid vectors are provided which enable for the modification of double-stranded DNA which has blunt or one-base 3' overhanging termini, to include any desired four-base 5' overhang sequence, in the reading frame of choice. These vectors enable the cloning of blunt double-stranded DNA sequences or double-stranded DNA sequences having a one-base 3' overhang into a unique restriction site by blunt-end ligation or by one-base 3' overhang cohesive cloning such as T-cloning. The vectors may be amplified and subsequently excised, thereby producing modified fragments free of vector sequences.