The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 20, 1998
Filed:
Jun. 07, 1995
Norman C Nelson, San Diego, CA (US);
Jorge Velarde, Jr, San Diego, CA (US);
Daniel L Kacian, San Diego, CA (US);
Gen-Probe Incorporated, San Diego, CA (US);
Abstract
The invention features a method for assaying the ability of an oligonucleotide to form a hybrid with a target nucleic acid sequence. The featured assay comprises the following steps: (a) contacting a test sample containing a target nucleic acid sequence with an oligonucleotide; (b) treating the test sample with a duplex-altering agent; (c) contacting the treated test sample with a labeled probe under stringent hybridization conditions, wherein the probe can hybridize to non-altered target nucleic acid sequence such that the probe label is protected from subsequent chemical inactivation but the probe cannot hybridize to altered target nucleic acid sequence in a manner which would protect the probe label from subsequent chemical inactivation; and (d) measuring the hybridization of the probe to non-altered target nucleic acid sequence by measuring the amount of protected label. Also described is an assay to measure the ability of a ribozyme to cleave a target nucleic acid sequence, and an assay to measure the ability of an agent to alter nucleic acid.