The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 22, 1998

Filed:

Jun. 11, 1997
Applicant:
Inventors:

Randolph L Rill, Tallahassee, FL (US);

Zhi-Ren Liu, Tallahassee, FL (US);

Assignee:

Florida State University, Tallahassee, FL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ; G01N / ;
U.S. Cl.
CPC ...
436 94 ; 436 86 ; 436175 ; 436 63 ;
Abstract

The novel dicationic dye N,N'-bis�3,3'-(dimethylamino)propylamine!-3,4,9,10-perylenetetracarboxylic diimide (DAPER) is prepared by reacting 3,4,9,10-perylenetetracarboxylic dianhydride with N,N-dimethylaminopropylamine. The dye DAPER is particularly useful in a rapid, sensitive method for the precipitation, recovery and quantitation of DNA from solutions containing DNA, which method involves the steps of (a) adding the dye DAPER to a solution containing DNA and allowing the resulting mixture to equilibrate; (b) collecting the resulting precipitate; (c) removing weakly bound DAPER from the precipitate; (d) extracting DAPER therefrom by the addition of a solvent therefor; and (e) determining the amount of DNA precipitated by measuring either the absorbance or fluorescence of DAPER in the solvent extract from step (d).


Find Patent Forward Citations

Rill, Randolph. (1998). Dicationic perylene dye for precipitation and quantitation of dna (U.S. Patent No. 5811307). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/5811307

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