Method of affinity purifying antibodies

Abstract

The present invention relates to a method of affinity purifying antibodies consisting of the following steps: A) homogenizing Helminth parasite worms forming a mixture; B) incubating the mixture of adult Helminth parasite worm homogenate with pre-swelled CNBr-activated Sepharose 4B in coupling buffer; C) first washing the mixture with blocking buffer; D) second washing the mixture with acetate buffer and coupling buffer; E) third washing the mixture with Phosphate Buffered Solution; F) pouring the mixture into a chromatography column; G) allowing the mixture to settle; H) first passing, by gravity, a solution of saturated ammonium sulfate precipitated antibodies in loading buffer through the chromatography column; I) second passing, by gravity, loading buffer through the chromatography column until thoroughly washed; and J) second passing, by gravity, chromatography column elution buffer through the chromatography column which functions to remove and the purified antibodies.