The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 11, 1998

Filed:

Jul. 06, 1995
Applicant:
Inventor:

Stephen Gwyn Ballard, Hamden, CT (US);

Assignee:

Yale University, New Haven, CT (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N / ;
U.S. Cl.
CPC ...
2504581 ; 2504591 ;
Abstract

An imaging scheme for illuminating an object having a plurality of fluors comprising: a light source for simultaneously illuminating the object with light having a plurality of separate excitation bands respectively falling within the fluorescent absorption bands of the fluors; and a detector positioned to receive fluorescent light from the fluors having wavelengths corresponding to the respective fluorescence emission bands of the fluors and adapted to produce a signal representative of the intensity of light received from the object. Also disclosed is an optical filtering scheme for receiving light having a first wavelength distribution and for providing light having a second selected wavelength distribution different from the first wavelength distribution. The above-mentioned imaging and filtering schemes enable simultaneous excitation and detection of a plurality of fluors, while preventing corruption by scattered or reflected excitation light. This enables efficient detection of about 90-95% of the fluorescence emissions from the fluors in the sample.


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