The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 28, 1998

Filed:

May. 02, 1997
Applicant:
Inventors:

Luigi Catanzariti, Duxbury, MA (US);

Bryan W Kluttz, Norwell, MA (US);

Geoff A McKinley, Duxbury, MA (US);

Arthur L Garland, Carver, MA (US);

Louis Graziano, Rockland, MA (US);

James G Moe, Franklin, MA (US);

Marcela Vera-Garcia, Framingham, MA (US);

James Clement Bishop, Columbia, MO (US);

David Chastain, Acton, MA (US);

Fabio Gennari, Florence, IT;

Assignee:

bioMerieux Vitek, Inc., Hazelwood, MO (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12M / ; G01N / ; C12N / ;
U.S. Cl.
CPC ...
435 911 ; 435 912 ; 435 915 ; 4352851 ; 4352872 ; 4352873 ; 4352876 ; 4352879 ; 4352882 ; 4352885 ; 4352861 ; 422 64 ; 422101 ; 422102 ; 935 78 ; 935 87 ; 935 88 ;
Abstract

A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix, a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber, the fluid channel is opened to allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction. A station is described for processing test strips incorporating the reaction vessels. The station includes temperature and vacuum control subsystems to maintain proper temperatures in the reaction vessel and effectuate the transfer of the fluid from one chamber to the other in an automated fashion without human intervention.


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