Immunoenzymatic detection device

Abstract

A device for detecting the presence of substances, particularly biological materials, contained in a liquid sample is formed of a series of layers of film materials. A first film has a cut-out which forms an upper reservoir for receiving the liquid sample. A first reagent is provided in the upper reservoir capable of reacting with a substance possibly contained in the liquid sample. A first membrane is situated at the base of the upper reservoir and is made of a material which is temporarily impermeable so that the liquid sample is temporarily retained in the upper reservoir in contact with the first reagent, but after awhile the membrane becomes permeable and allows the liquid to pass. A second film has a cut-out which forms a median reservoir positioned for receiving the liquid passing through the first membrane. A second reagent is provided in the median reservoir capable of reacting with a predetermined substance. A second membrane is situated at the base of the second reservoir and is similarly made of a material which is temporarily impermeable, but which later becomes permeable. A third film has a cut-out forming a lower reservoir positioned for receiving the liquid passing through the second membrane and a third reagent is provided in the lower reservoir and is formed of a material capable of developing a color reaction by contact with the liquid which reaches the lower reservoir after passing through the second membrane.