The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 1998
Filed:
May. 23, 1995
Bruce A Green, Pittsford, NY (US);
Gary W Zlotnick, Penfield, NY (US);
Praxis Biologics, Inc., Rochester, NY (US);
Abstract
'A method of purifying protein 'e' from Haemophilus influenzae includes disrupting H. influenzae cells, subjecting the disrupted cells by differential sedimentation to obtain a total cell membrane fraction, fractionating the total cell membrane into inner and outer membrane components by density gradient sedimentation or by differential solubilization of the inner membrane component with detergents, obtaining a subfraction of the preparation of the outer membrane components which is enriched in protein 'e' by extraction with an aqueous solution of 0.1-2.0% N-lauroyl sarcosine, sodium salt, solubilizing the protein 'e' from the subfraction by a two-step differential solubilization process with sulfobetaine detergents, and recovering the aqueous solution which contains the purified protein 'e'.