The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 14, 1998

Filed:

Nov. 14, 1996
Applicant:
Inventors:

Stephen A Livesey, Victoria, AU;

Anthony A del Campo, Houston, TX (US);

Abhijit Nag, Houston, TX (US);

Ken B Nichols, Houston, TX (US);

Carmen Piunno, The Woodlands, TX (US);

David P Ross, Houston, TX (US);

Assignee:

Life Cell Corporation, The Woodlands, TX (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M / ;
U.S. Cl.
CPC ...
4353071 ; 435260 ; 4352831 ; 62346 ; 62347 ; 34 62 ;
Abstract

This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in an appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants.


Find Patent Forward Citations

Loading…