The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 1998
Filed:
Oct. 06, 1995
Applicant:
Inventors:
Honghua Li, Cherry Hill, NJ (US);
Yong-Sheng Xu, Haddonfield, NJ (US);
Assignee:
Coriell Institute for Medical Research, Camden, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12N / ;
U.S. Cl.
CPC ...
435-6 ; 435259 ; 4353171 ;
Abstract
A composition is provided, which comprises a purified cytoplasmic extract from eggs of Xenopus laevis which is capable of supporting activation of human sperm and complete replication of a human sperm genome. Methods of using the extract to achieve complete replication of the sperm genome are disclosed. A flow cytometric method for quantitatively monitoring replication of human sperm genomes is also disclosed. This method can be used for clinical assessment of sperm quality in a test sample of human sperm from an infertile male donor.