The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 07, 1998

Filed:

Jan. 30, 1996
Applicant:
Inventors:

James G Paulson, Del Mar, CA (US);

Eryn Ujita-Lee, Redondo Beach, CA (US);

Karen J Colley, Oak Park, IL (US);

Beverly Adler, Newbury Park, CA (US);

Jeffrey K Browne, Camaville, CA (US);

Jasminder Weinstein, Westlake Village, CA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
4353201 ; 435 41 ; 435 691 ; 435 701 ; 4351723 ; 435 97 ; 435197 ; 435100 ; 435325 ; 935 48 ; 935 60 ; 935 70 ; 935 72 ; 935 47 ; 536 235 ;
Abstract

A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.


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