The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 30, 1998
Filed:
Jun. 10, 1996
Daniel Sinnett, Boucherville, CA;
Damian LaBuda, Monteal, CA;
Maja Krajinovic, Monteal, CA;
Chantal Richer, Montre, CA;
Centre de recherche de l'Hopital Sainte-Justine, Montreal, CA;
Abstract
The present invention relates to a method of diagnosis or prognosis of tumor cells in a patient displaying a mutator phenotype in the tumor which comprises the steps of: a) obtaining a genomic DNA sample of the tumor; b) obtaining a genomic DNA sample of a tumor-free tissue of the patient; subjecting the DNA samples of steps a) and b) to amplification using primers which are flanking repeat pattern affected by a mutator phenotype or cold inter-Alu PCR followed by hybridization with a probe corresponding to an instability prone locus; d) subjecting the amplified fragments of step c) to electrophoretic fractionation on a polyacrylamide gel to determine the presence of a variation in band profile between tumor and tumor-free DNA indicating a genomic instability associated with a mutator phenotype; and e) employing means for comparing the allelic status of at least one instability prone locus in the DNA samples of step c).