The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 09, 1998
Filed:
Jan. 17, 1997
Applicant:
Inventors:
Alan P Sloma, Davis, CA (US);
William R Widner, Davis, CA (US);
Assignee:
Novo Nordisk Biotech, Inc., Davis, CA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ; C12N / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 435 712 ; 43525231 ; 4352525 ; 4353201 ; 536 237 ;
Abstract
The present invention relates to a modified bacterial donor cell useful in conjugation, the cell containing i) a plasmid comprising a DNA construct encoding the polypeptide of interest, and at least one cis-acting DNA sequence required for the transfer of said plasmid by conjugation in the presence of a trans-acting mobilizing element, and ii) at least one DNA sequence encoding said trans-acting mobilizing element, wherein the donor cell has a reduced capacity, relative to a parent cell, of producing a bactericidal agent which kills or prevents growth in a population of recipient cells.